Method for purification of soluble ssao

C - Chemistry – Metallurgy – 12 – P

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C12P 21/02 (2006.01) C12N 9/06 (2006.01) C12N 15/12 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2433408

The present invention relates to a recombinant construct comprising a nucleotide sequence encoding a fusion protein comprising a soluble form a human SSAO (Semicarbazide-Sensitive Amine Oxidase), a secretable fusion partner, a signal peptide; and a protease cleavage site. The said construct is useful in methods for purification of a soluble form of human SSAO.

La présente invention concerne une construction de recombinaison comprenant une séquence nucléotidique codant pour une protéine hybride comprenant une forme soluble d'une amine oxydase sensible au semicarbazide (SSAO) humaine, un partenaire d'hybridation pouvant être sécrété, un peptide signal; et un site de restriction de protéase. Ladite construction est utile dans des procédés permettant de purifier une forme soluble de SSAO humaine.

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