Method for purification of viral vectors having proteins...

C - Chemistry – Metallurgy – 12 – N

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12N 15/864 (2006.01) C07K 14/015 (2006.01) C12N 7/01 (2006.01) C12N 7/02 (2006.01) C12N 15/63 (2006.01) C12P 1/04 (2006.01)

Patent

CA 2455499

A method for isolating, from a mixture, a virus having a surface protein with a binding site for sialic acid is provided. The method involves contacting the mixture with mucin which has been linked to a solid support and washing the solid support to remove material from the mixture which is non-specifically bound to the mucin- linked support. Thereafter, the specifically bound virus (e.g., AAV4 or AAV5) may be removed in a further washing step utilizing a concentrated salt or solution with low pH. Also described are pharmaceutical kits containing solid supports linked to mucin for use in isolating virus having a surface protein with a binding site for sialic acid, or detecting the presence of the virus in a biological sample.

Procédé visant à isoler, à partir d'un mélange, un virus possédant une protéine de surface ayant un site de liaison avec l'acide sialique. Ce procédé consiste à mettre en contact le mélange avec la mucine qui a été liée à un support solide et à laver le support solide pour éliminer le matériau du mélange qui est lié de manière non spécifique au support lié à la mucine. Par la suite, le virus lié de manière spécifique (tel que AAV4 ou AAV5) peut être éliminé dans une autre étape de lavage utilisant une solution ou un sel concentré à pH faible. L'invention porte également sur des kits pharmaceutiques contenant des supports solides liés à la mucine et destinés à être utilisés dans l'isolement du virus possédant une protéine de surface pourvue d'un site de liaison à l'acide sialique ou la détection du virus dans un échantillon biologique.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for purification of viral vectors having proteins... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for purification of viral vectors having proteins..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for purification of viral vectors having proteins... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1767611

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.