Method for purifying a physiologically active substance...

C - Chemistry – Metallurgy – 12 – N

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530/7.1

C12N 15/00 (2006.01) C07K 14/525 (2006.01)

Patent

CA 1265650

ABSTRACT OF THE DISCLOSURE An aqueous solution containing a physiologically active substance, which is produced by recombinant DNA technique and which has cytotoxic activity against L-M cells and is capable of inducing hemorrhagic necrosis of transplanted Meth A sarcoma in the BALB/c mouse, can be effectively, efficiently purified by column chromatography using a column packed with a dye-bonded crosslinked agarose gel.

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