Method for purifying an enzyme and purified enzyme produced...

Details Method for purifying an enzyme and purified enzyme produced... Method for purifying an enzyme and purified enzyme produced...

C - Chemistry, Metallurgy

12

N

C12N 9/00 (2006.01) C12N 9/52 (2006.01)

Patent

CA 2447818

The invention relates to a method for purifying at least one enzyme obtained in an excess fermentation of Clostridium histolyticum. It is provided for that the enzymes of the excess fermentation are separated by a multistage chromatography method by exclusively using chromatography materials on styrene/divinylbenzene base and/or on base of in particular ceramic hydroxylapatite.

L'invention concerne un procédé de purification d'au moins une enzyme contenue dans un surnageant de fermentation de Clostridium histolyticum. Selon ce procédé, les enzymes du surnageant de fermentation sont séparées par un procédé chromatographique en plusieurs étapes, consistant à utiliser exclusivement des matériaux chromatographiques à base de styrol/divivylbenzol et/ou à base d'hydroxylapatite, en particulier céramique.

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