Method for purifying fibroblast growth factor protein

Details Method for purifying fibroblast growth factor protein Method for purifying fibroblast growth factor protein

C - Chemistry, Metallurgy

12

N

530/13, 530/3.14

C12N 15/18 (2006.01) C07K 1/22 (2006.01) C07K 14/50 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2005154

27580-42 TITLE: METHOD FOR PURIFYING FIBROBLAST GROWTH FACTOR PROTEIN ABSTRACT Disclosed is a method for purifying a fibroblast growth factor (FGF) protein with use of a crosslinked polysaccharide sulfate. The FGF protein is preferably a mutein, in which at least one human basic FGF-constituent amino acid is substituted by at least one different amino acid. The crosslinked polysaccharide sulfate is preferably a crosslinked cellulose sulfate, a crosslinked agarose sulfate or a crosslinked dextran sulfate. According to the present invention, FGF can be highly purified on a commercial scale, and pharmaceutical preparations containing the FGF protein can be advantageously formulated.

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