Method for purifying fsh

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/59 (2006.01) A61K 38/04 (2006.01)

Patent

CA 2579131

The invention relates to a method for purifying recombinant human FSH or an FSH variant starting from crude FSH, comprising the following steps : 1. dye- affinity chromatography; 2. hydrophobic interaction chromatography; and 3. reverse phase chromatography .

La présente invention décrit une méthode de purification de la FSH humaine recombinante ou d'une variante de FSH. Ladite méthode a pour point de départ la FSH brute et comprend les étapes suivantes : 1. chromatographie d~affinité sur colorants immobilisés ; 2. chromatographie d~interaction hydrophobe ; et 3. chromatographie en phase inverse.

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