Method for purifying fsh or a fsh mutant

Details Method for purifying fsh or a fsh mutant Method for purifying fsh or a fsh mutant

C - Chemistry, Metallurgy

07

K

C07K 1/36 (2006.01) C07K 14/00 (2006.01) C07K 14/59 (2006.01)

Patent

CA 2625978

The invention relates to a method for purifying a glycoprotein, preferably FSH or a FSH mutant comprising the steps of subjecting a liquid containing said FSH or a FSH mutant to: (1) a dye affinity chromatography; (2) a weak anion exchange chromatography (3) a hydrophobic interaction chromatography; and (4) a strong anion exchange chromatography ; which may be carried out in any order.

L~invention concerne un procédé de purification d~une glycoprotéine, de préférence la FSH ou un mutant de la FSH, comprenant les étapes consistant à soumettre un liquide contenant ladite FSH ou un mutant de la FSH à : (1) une chromatographie d~affinité tinctoriale ; (2) une chromatographie d~échange anionique faible ; (3) une chromatographie d~interaction hydrophobe et (4) une chromatographie d~échange anionique fort qui peuvent être réalisées dans n~importe quel ordre.

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