Method for purifying gamma-interferon

C - Chemistry – Metallurgy – 07 – K

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167/103.1

C07K 14/57 (2006.01) C07K 16/24 (2006.01)

Patent

CA 1237669

ABSTRACT OF THE DISCLOSURE A method is provided for restoring some or all of the activity of gamma-interferon which has been in con- tact with an acidic solution comprising the steps of: a) placing the gamma-interferon in a solution which has a pH between about 5.5 and 9.5; and b) incubating the solution at a temperature of between about 2°C and 8°C for a period of at least 24 hours. The method allows antibody affinity chromatography employing acid elution to be used to purify gamma-interferon, in that, the ac- tivity of the acid-eluted gamma-interferon can be es- sentially completely restored using the reactivation process.

469662

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