Method for purifying granulocyte colony stimulating factor

Details Method for purifying granulocyte colony stimulating factor Method for purifying granulocyte colony stimulating factor

C - Chemistry, Metallurgy

07

K

C07K 14/535 (2006.01) C07K 1/20 (2006.01)

Patent

CA 2378566

The invention concerns a method for purifying G-CSF from a biological sample comprising steps which consist in: a) reducing the volume of the biological sample containing the G-CSF by hydrophobic interaction chromatography to obtain a concentrated, desalted and enriched fraction; b) passing the concentrated fraction over hydroxyapatite in conditions whereby the G-CSF is slightly bound to obtain a concentrated, desalted and enriched fraction containing the G-CSF; and c) collecting the G-CSF.

L'invention a pour objet un procédé de purification de G-CSF à partir d'un échantillon biologique comprenant les stades de: a) réduire le volume de l'échantillon biologique contenant le G-CSF par chromatographie par interaction hydrophobe pour obtenir une fraction concentrée, dessalée et enrichie; b) passer la fraction concentrée sur hydroxyapatite dans les conditions où le G-CSF est faiblement lié pour obtenir une fraction concentrée, dessalée et enrichie contenant le G-CSF c) et; recueillir le G-CSF.

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