Method for purifying or detecting a target protein

Details Method for purifying or detecting a target protein Method for purifying or detecting a target protein

C - Chemistry, Metallurgy

07

K

C07K 1/22 (2006.01) C07H 21/00 (2006.01) C07K 1/14 (2006.01) C07K 14/745 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2696363

The invention relates to a method for purifying or detecting a target protein present in a solution, wherein said method comprises, before carrying out the purification or detection step itself, the step of contacting said solution with an aptamer binding specifically to said target protein, wherein said aptamer does not bind to any protein homologous to said target protein that could also be present in the solution.

L'invention a pour objet un procédé pour la purification ou la détection d'une protéine cible présente dans une solution, ledit procédé comprenant, préalablement à la réalisation de l'étape de détection ou de purification proprement dite, une étape de mise en contact de ladite solution avec un aptamère se liant spécifiquement à ladite protéine cible, ledit aptamère ne se liant pas à une protéine homologue à ladite protéine cible susceptible d'être également présente en solution.

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