Method for purifying polypeptides

Details Method for purifying polypeptides Method for purifying polypeptides

C - Chemistry, Metallurgy

07

K

C07K 14/65 (2006.01) C07K 1/20 (2006.01) C07K 1/22 (2006.01) C07K 14/475 (2006.01) C07K 14/575 (2006.01)

Patent

CA 2177575

A process is provided for selectively separating a polypeptide of interest from components of differing in a mixture comprising the steps of: a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the silica particles; b) washing the silica particles to remove impurities; and c) eluting the polypeptide from the silica particles with a buffer comprising an alcoholic or polar aprotic solvent and an alkaline earth, an alkali metal, or an inorganic ammonium salt,

Un procédé pour séparer de manière sélective un polypeptide, présentant un intérêt, de composants d'hydrophobie différente dans un mélange, consiste (a) à passer le mélange à travers des particules de silice non dérivées de façon à ce que le polypeptide adhère aux particules de silice; (b) à laver les particules de silice afin d'éliminer les impuretés et (c) à éluer le polypeptide des particules de silice avec une solution tampon comprenant un solvant aprotique polaire ou alcoolisé et de la terre alcaline, un métal alcalin, ou un sel d'ammonium minéral.

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