Method for purifying proteins

C - Chemistry – Metallurgy – 40 – B

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Details

C40B 40/04 (2006.01) C40B 40/08 (2006.01) C40B 40/10 (2006.01)

Patent

CA 2602913

The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample.

L'invention concerne des procédés et des nécessaires destinés à purifier un groupe de protéines cibles. Le procédé comprend les étapes consistant à mettre en contact un échantillon contenant au moins 95 % du groupe de protéines cibles et au plus 5 % de protéines contaminantes avec une bibliothèque de groupes fonctionnels de liaison possédant des groupes fonctionnels de liaison différents, liant les protéines contaminantes et une minorité du groupe de protéines cibles à la librairie de groupes fonctionnels de liaison, séparant le groupe de protéines cibles non liées des protéines liées à la bibliothèque de groupes fonctionnels de liaison et collectant la protéine cible non liée. La protéine cible collectée est plus pure que le groupe de protéines cibles de l'échantillon.

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