Method for purifying the factor viii and the von willebrand...

C - Chemistry – Metallurgy – 07 – K

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C07K 1/18 (2006.01) A61K 38/36 (2006.01) A61K 38/37 (2006.01) A61P 7/04 (2006.01) C07K 14/55 (2006.01) C07K 14/745 (2006.01) C07K 14/755 (2006.01)

Patent

CA 2697404

The invention relates to a purification method that comprises, from a solution selected from (i) a solution containing a mixture of FVIII and FvW, (ii) a solution containing FvW, (iii) a solution resulting from a secretion of a non-human animal and (iv) a solution resulting from a vegetable extract containing FVIII, the step of absorbing the FVIII or the FvW on an ion-exchange chromatography filtration membrane.

Le procédé de purification comprend, à partir d'une solution choisie parmi (i) une solution contenant un mélange de FVIII et FvW, (ii) une solution contenant du FvW, (iii) une solution issue d'une sécrétion d'un animal non-humain et (iv) une solution issue d'un extrait végétal contenant du FVIII, une étape d'absorption du FVIII ou du FvW sur une membrane filtrante de chromatographie échangeuse d'ions.

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