C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 14/52 (2006.01) C07K 1/16 (2006.01)
Patent
CA 2223236
Methods of removing protein contaminants from a solution comprising thrombopoietin are disclosed. The solution is exposed to hydroxyapatite, whereby protein contaminants are bound to the hydroxyapatite and the thrombopoietin remains substantially unbound, and the unbound thrombopoietin is recovered. The use of hydroxyapatite is advantageously combined with other purification and concentration techniques, including ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatography, ultrafiltration, and differential precipitation.
L'invention concerne des procédés visant à extraire des contaminants protéiniques d'une solution contenant des thrombopoïétines. La solution est exposée à une hydroxyapathite. Ainsi, les contaminants protéiniques sont liés à l'hydroxyapathite, tandis que les thrombopoïétines restent pratiquement non liées, ce qui permet de les récupérer. L'utilisation de l'hydroxyapathite est combinée avantageusement avec d'autres techniques de purification et de concentration, y compris la chromatographie d'échange d'ions, la chromatographie d'affinité de ligand, la chromatographie d'interaction hydrophobe, l'ultrafiltration et la précipitation sélective.
Alaska Andrew R.
Chang Jin-Jyi
Downey William
Forstrom John W.
Phan Linh
Ledgley Law
Zymogenetics Inc.
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