Method for purifying virus

Details Method for purifying virus Method for purifying virus

C - Chemistry, Metallurgy

12

N

C12N 7/02 (2006.01)

Patent

CA 2529053

A process for the purification of viruses from a cell lysate preparation is described, consisting of preferably two successive chromatographic steps; the first a clarification step utilizing size exclusion chromatography, and the second, a virus capture and release step using anion exchange chromatography, which successive chromatographic steps have the advantage of purifying virus, and avoiding chromatography buffer conductivity adjustments.

L'invention concerne un procédé destiné à la purification de virus à partir d'une préparation de lysat cellulaire, et comprenant de préférence deux opérations chromatographiques successives. La première est une opération de clarification faisant appel à une chromatographie d'exclusion de taille, la seconde étant une opération de capture et de libération de virus faisant appel à une chromatographie d'échange d'anions, ces opérations chromatographiques successives permettant avantageusement de purifier un virus et d'éviter les réglages de conductivité de tampon de chromatographie.

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