C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/123, 195/78
C12N 9/42 (2006.01) C07K 3/26 (1990.01)
Patent
CA 1283877
ABSTRACT OF THE DISCLOSURE The separation of xylanases from mixtures thereof with other hemcellulases, particulary cellulase produced by the cul- turing of hemicellulolytic microorganisms, particularly the fungus Trichoderma harzianum E58 and Trichoderma reesei by ultrafiltration through an ultrafiltration membrane having a low molecular weight cut-off point in the range of about 1,000 to 20,000 daltons to obtain a cellulase rich retentate and xylanase rich ultrafiltrate. The dilute xylanase rich filtrate from the ultrafiltration is concentrated and purified by adsorption and elution from an insoluble matrix, e.g. a cationic exchange resin. The xylanase obtained is suitable for use in the hydro- lysis of hemicellulose for which it is selective, particularly in the presence of cellulose and the cellulase rich retentate is suitable for the hydrolysis of cellulose.
558529
Saddler John N.
Tan Larry U.l.
Yu Ernest K.c.
Forintek Canada Corp.
Perley-Robertson Hill & Mcdougall
Saddler John N.
Tan Larry U.l.
Yu Ernest K.c.
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