Method for qualitative and/or quantitative determination of...

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G01N 33/483 (2006.01) G01N 27/62 (2006.01) G01N 33/68 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2452851

The invention relates to a method for determining the gender, species, race and/or geographical origin of biological materials. Fibrillary structures such as feathers, down, scales, hair or horn are directly cleaved in an enzymatic and specific manner to form a pool of cleaved peptides, without previously rendering structure proteins soluble. A total hydrolysis forming amino acids is not carried out, neither is a special analyte sought. The pool of cleaved peptides obtained from fibrillary structure proteins is preferably subjected to MALDI-ToF-mass spectroscopy, without using any other separating or isolating techniques, and the mass spectrograms obtained are adjusted using reference mass spectrograms. Suitable specific mass peaks are used to quantify elements such as feathers, down, scales, horn and/or hair which are foreign to the species.

L'invention concerne un procédé permettant de déterminer le genre, l'espèce, la race et/ou l'origine géographique de matériaux biologiques. A cet effet des structures fibrillaires telles que des plumes, des duvets, des écailles, des poils ou de la corne sont, sans qu'il soit nécessaire de préalablement rendre des protéines de structures solubles, soumis à un clivage direct, de façon enzymatique et spécifique, pour constituer un ensemble de peptides de clivage. Pour cela on ne procède ni à une hydrolyse totale résultant en aminoacides, ni à la recherche d'un analyte spécial. L'ensemble de peptides de clivage obtenu à partir des protéines de structures fibrillaires est soumis, sans mise en oeuvre d'autres techniques de séparation ou d'isolation, à une spectroscopie de masse MALDI-ToF, et les spectrogrammes de masse obtenus sont ajustés avec des spectrogrammes de masse de référence. Des pics de masse spécifiques appropriés sont exploités pour la quantification d'éléments tels que les plumes, les duvets, les écailles, la corne et/ou les poils, ajoutés étrangers à l'espèce.

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