Method for quantification of an analyte

Details Method for quantification of an analyte Method for quantification of an analyte

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/09 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/06 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G01N 33/58 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2302881

The invention provides a new method for the quantification of an analyte, wherein in a first step, the analyte is amplified by an amplifying agent. Subsequently, the amount of amplification product is determined as a function of time and the extremae of first, second or n th order derivatives are calculated. The obtained values can then be used to calculate the initial concentration of the analyte.

L'invention prévoit une nouvelle méthode pour déterminer la quantité d'un analyte. Comme première étape, l'analyte est amplifié par un agent amplifiant. Ensuite, la quantité du produit d'amplification est déterminé en fonction du temps et de l'extrême des premier, deuxième ou n ordre de dérivés qui sont calculés. Les valeurs obtenues peuvent ensuite être utilisées pour calculer la concentration initiale d'analyte.

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