Method for quantification of biological material in a sample

Details Method for quantification of biological material in a sample Method for quantification of biological material in a sample

C - Chemistry, Metallurgy

12

Q

C12Q 1/06 (2006.01) B01L 3/00 (2006.01) C12M 1/20 (2006.01) C12M 1/22 (2006.01)

Patent

CA 2237639

Method for detection of a biological material in a sample. The method includes the steps of liquefying the sample (if necessary) and pouring the liquefied sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquefied sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.

Ce procédé sert à détecter une substance biologique dans un échantillon. Ce procédé consiste à liquéfier l'échantillon (si nécessaire) et à verser l'échantillon ainsi liquéfié dans la plaque d'incubation. Cette plaque d'incubation possède une surface horizontale généralement plate et cette surface est divisée en au moins 20 puits creux. Chaque puits est conçu pour contenir une aliquote d'un liquide et sa grandeur et sa forme ainsi que sa constitution en un matériau approprié sont telles qu'il peut contenir l'aliquote grâce à sa tension de surface. Tout liquide excédentaire provenant de l'échantillon liquéfié est versé depuis la surface de la plaque. Ledit procédé consiste ensuite à incuber cette plaque d'incubation jusqu'à ce que la présence ou l'absence de la substance biologique soit déterminée.

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