C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2750029
The present invention relates to a method for amplification and quantification of mscroRNA molecules using quantitative reverse transcription polymerase chain reaction (q RT- PC R) technology. The method comprise the steps of (a) producing cDNA molecules complementary' to microRNAs in a sample using polyadenylation and primer extension by reverse transcription, and (b) amplification and quantification of the cDNAs by qPCR using microRNA specific primer sets of forward and reverse primers containing LNA monomers.
La présente invention concerne un procédé pour l'amplification et la quantification de molécules de microARN mettant en uvre la technologie quantitative de transcription inverse- la réaction en chaîne de la polymérase (q RT- PCR). Le procédé comprend les étapes suivantes : (a) la production de molécules ADNc complémentaires aux microARN dans un échantillon au moyen d'une polyadénylation et d'une extension d'amorce par la transcription inverse, et (b) l'amplification et la quantification des ADNc par la technologie qPCR au moyen d'ensembles d'amorces spécifiques de microARN d'amorces avant et arrière contenant des monomères LNA.
Exiqon A/s
Ridout & Maybee Llp
LandOfFree
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