Method for quantifying denatured ldl

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 19/00 (2006.01) A61K 38/17 (2006.01) C07K 14/705 (2006.01) C07K 17/00 (2006.01) C12N 15/62 (2006.01) G01N 33/53 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2315280

An assay method commonly usable in the clinical field whereby denatured LDLs such as oxidized LDL contained in the body fluids of patients with arteriosclerosis, hyperlipemia, etc. can be conveniently detected and quantitated in an intact state at a high sensitivity; and a fused polypeptide to be used as a constituent in the above assay method, which is composed of the extracellular region of a mammalian oxidized LDL receptor and a part of the heavy chain of a mammalian immunoglobulin. This fused polypeptide per se is useful as the active ingredient in drugs for preventing and treating arteriosclerosis, hyperlipemia, etc.

L'invention concerne un procédé de dosage qui peut être utilisée de manière courante dans le domaine clinique. Ce procédé permet de doser facilement les LDL dénaturées telles que des LDL oxydées contenues dans les fluides corporels des patients souffrant d'artériosclérose, d'hyperlipémie etc., dans un état intact et avec une haute sensibilité. L'invention concerne également un polypeptide fusionné qui peut être utilisé comme constituant du procédé ci-dessus. Ce polypeptide est composé de la région extracellulaire d'un récepteur LDL oxydé de mammifère et d'une partie de la chaîne lourde d'une immunoglobuline de mammifère. Par ailleurs ce polypeptide fusionné est utile comme ingrédient actif dans des médicaments servant à prévenir et à traiter l'artériosclérose, l'hyperlipémie etc.

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