Method for quantitating oligonucleotides

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 19/00 (2006.01) C07H 21/00 (2006.01) C07H 21/02 (2006.01)

Patent

CA 2391004

Methods and processes for quantitating the amount of oligonucleotide in a sample of bodily fluid and/or extract are provided. The methods and processes provided allow for the detection and/or localization of oligonucleotides for therapeutic and/or pharmacokinetic purposes by providing a highly sensitive assay that takes advantage of enzymes which recognize specific nucleic acid conformations and structures.

La présente invention concerne des méthodes et des procédés permettant de quantifier la quantité d'oligonucléotides présents dans un échantillon de fluide et/ou extrait corporel. Les méthodes et procédés de l'invention permettent la détection et/ou la localisation d'oligonucléotides à des fins thérapeutiques et/ou pharmacocynétiques grâce à un test à sensibilité élevée qui exploite la capacité d'enzymes à reconnaître des conformations et structures d'acide nucléique spécifiques.

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