Method for quantitating triglycerides in lipoproteins

Details Method for quantitating triglycerides in lipoproteins Method for quantitating triglycerides in lipoproteins

C - Chemistry, Metallurgy

12

Q

C12Q 1/61 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2360679

A method for conveniently quantitating a TG in various lipoproteins. This method is characterized by eliminating free glycerol from a sample containing free glycerol and a TG in a specific lipoprotein, treating the residue with lipoprotein lipase and an enzymatic system whereby hydrogen peroxide is formed from free glycerol, and then quantitating the hydrogen peroxide thus formed to thereby quantitate the TG in the specific lipoprotein.

L'invention concerne une méthode qui permet de mesurer sans inconvénient un triglycéride dans diverses lipoprotéines. Le procédé consiste à éliminer un glycérol libre d'un prélèvement contenant en outre un triglycéride dans une lipoprotéine spécifique. Le procédé consiste ensuite à traiter le résidu avec une lipoprotéine-lipase et un système enzymatique afin de former un peroxyde d'hydrogène à partir dudit glycérol libre. Le procédé consiste enfin à mesurer le peroxyde d'hydrogène ainsi formé pour mesurer le triglycéride dans la lipoprotéine spécifique.

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