C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2319709
The invention relates to a method for quantitatively analyzing tumor cells in a body fluid. According to the inventive method, the test sample to be examined is first subjected to a method for accumulating or depleting tumor cells, and a reaction is carried out on the accumulated or depleted tumor cells. During the reaction, the mRNA which codes for the catalytic subunit of the telomerase is specifically amplified, and afterwards, the quantity of amplified nucleic acid is quantitatively analyzed. The invention also relates to test kits suited for the invented method.
L'invention concerne un procédé pour la quantification des cellules tumorales dans un fluide corporel. Selon ce procédé, l'échantillon à analyser est tout d'abord soumis à un procédé visant à l'enrichissement ou à l'appauvrissement des cellules tumorales, et une réaction est effectuée sur ces cellules tumorales enrichies ou appauvries. Au cours de cette réaction, l'ARNm codant pour la sous-unité catalytique de la télomérase est spécifiquement amplifié, et ensuite, la quantité d'acide nucléique amplifiée fait l'objet d'une analyse quantitative. L'invention concerne également des trousses d'essai appropriées à cet effet.
Brockmeyer Carsten
Dahm Michael W.
Phelps Robert C.
Dahm Michael W.
Goudreau Gage Dubuc
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