Method for quantitatively assaying protein c and activator...

Details Method for quantitatively assaying protein c and activator... Method for quantitatively assaying protein c and activator...

G - Physics

01

N

167/43, 150/15.3

G01N 33/68 (2006.01) A61K 35/58 (2006.01) C12Q 1/37 (2006.01) C12Q 1/56 (2006.01)

Patent

CA 1286223

A B S T R A C T A method for assaying protein C is described, which comprises reacting a protein C-containing medium with venom of the snake Agkistrodon contortrix, or venom of another snake species which undergoes an immunologi- cal cross-reaction with the venom of Agkistrodon contor- trix, or a protein C-activating activator preparation obtained from one of the said venoms for a period of ti- me sufficient to cause maximum activation of protein C and subsequently determining the quantity of activated protein C thus formed either by photometrically measu- ring the quantity of coloured or fluorescent split pro- duct formed as a result of the catalytic hydrolytic ac- tion of activated protein C on a synthetic chromogenic substrate, or by measuring the prolongation of the clot- ting time of a natural substrate caused by activated protein C.

510137

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