Method for quantitatively determining the level of...

G - Physics – 01 – N

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150/11

G01N 33/72 (2006.01)

Patent

CA 1213199

ABSTRACT OF THE DISCLOSURE A method of quantitatively determining the amount of hemoglobin in a biological material which includes the steps of preparing a test sample of the biological material by removing contaminating elements such as sources of interfering fluorescence, converting the hemoglobin in the test sample to porphyrin, performing an extraction step for the purpose of separating the porphyrins derived from hemoglobin from various contaminants and determining the level of porphyrin in the test sample by means such as fluorescence assay.

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