C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/02 (2006.01) G01N 33/573 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2074507
2074507 9111714 PCTABS00006 A solid phase immunoassay using a natural protease substrate as solid phase to capture a metalloproteinase enzyme capable of degrading gelatin and type IV collagenase is provided. Gelatin is bound to a solid substrate and soluble type IV collagenase of interest is introduced. After washing and introduction of affinity purified, rabbit anti-peptide antibodies against type IV collagenase followed by goat anti-rabbit-peroxidase conjugate, the amount of bound enzyme is determined.
Liotta Lance A.
Stetler-Stevenson William G.
Gowling Lafleur Henderson Llp
U.s. Department Of Commerce United States Of America Represented By The Secretary
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