Method for rapid base sequencing in dna and rna

Details Method for rapid base sequencing in dna and rna Method for rapid base sequencing in dna and rna

C - Chemistry, Metallurgy

12

Q

195/1.12

C12Q 1/68 (2006.01)

Patent

CA 1314247

METHOD FOR RAPID BASE SEQUENCING IN DNA AND RNA ABSTRACT OF THE INVENTION A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identifica- tion. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.

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