Method for rapid detection of bacterial growth in cultures

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01)

Patent

CA 2291476

This invention provides a method for growth detection and identification of microorganisms in a culture medium comprising providing a culture medium comprising a solubilized oxygen-quenchable phosphorescent compound, inoculating said culture medium with a substrate suspected of being associated with one or more microorganisms, and detecting microorganism growth and identifying said microorganism by causing said phosphorescent compound to phosphoresce and observing quenching of oxygen in said culture of said compound.

Cette invention concerne un procédé de détection de croissance bactérienne et d'identification de microorganismes dans un milieu de culture. Ce procédé consiste à utiliser un milieu de culture comprenant un composé phosphorescent solubilisé dont la phosphorescence peut être atténuée par de l'oxygène, à inoculer ledit milieu de culture à un substrat suspecté d'être associé à un ou plusieurs microorganismes; puis à détecter la croissance du microorganisme, à identifier ce dernier en rendant phosphorescent ledit composé phosphorescent et à observer l'effet atténuateur de l'oxygène dans le milieu de culture dudit composé.

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