Method for readily detecting class c...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/04 (2006.01)

Patent

CA 2549697

A method of judging whether a microorganism to be tested is a strain producing class C .beta.-lactamase or not. A class C .beta.-lactamase inhibitor and a .beta.-lactam agent are placed at a definite interval over the surface of a solid medium which is coated with the microorganism to be tested. Then the solid medium is cultured. After culturing, judgment is made based on whether or not an inhibitory zone formed around the .beta.-lactam agent is enlarged toward the class C .beta.-lactamase inhibitor side. A mixture of a class C .beta.-lactamase inhibitor with a .beta.-lactam agent and a .beta.-lactam agent are placed at a definite interval over the surface of a solid medium which is coated with the microorganism to be tested. Then the solid medium is cultured. After culturing, judgment is made based on the difference between the inhibitory zone formed around the mixture and the inhibitory zone formed around the .beta.-lactam agent. A judgment kit wherein a disc containing a class C .beta.-lactamase inhibitor and a disc containing a .beta.-lactam agent are provided on a base strip. A judgment kit wherein a disc containing a class C .beta.-lactamase inhibitor with a .beta.-lactam agent and a disc containing a .beta.-lactam agent are provided on a base strip. A method of conveniently detecting class C .beta.-lactamase and a kit for the embodiment of this method.

L'invention porte sur un procédé qui permet de déterminer si un organisme à tester est une souche produisant la beta -lactamase de classe C ou non. Selon l'invention, on place un inhibiteur de beta -lactamase de classe C et un agent beta -lactame à un intervalle défini à la surface d'un support solide qui est recouvert du micro-organisme à tester. On met ensuite le support solide en culture. Après la culture, on détermine si une zone inhibitrice formée autour de l'agent beta -lactame s'est élargie du côté de l'inhibiteur de beta -lactamase de classe C. On place un mélange d'un inhibiteur de beta -lactamase de classe C et d'un agent beta -lactame et un agent beta -lactame à un intervalle défini à la surface d'un support solide qui est revêtu du micro-organisme à tester. On met ensuite le support solide en culture. Après la culture, on compare la différence entre la zone inhibitrice formée autour du mélange et la zone inhibitrice formée autour de l'agent beta -lactame. L'invention concerne une trousse de détection comprenant un disque contenant un inhibiteur de beta -lactamase de classe C et un disque contenant un agent beta -lactame disposés sur un ruban support. L'invention se rapporte à une trousse de détection comprenant un disque contenant un inhibiteur de beta -lactamase de classe C et un agent beta -lactame, et un disque contenant un agent beta -lactame disposés sur un ruban support. L'invention porte enfin sur un procédé qui permet de détecter facilement une beta -lactamase de classe C, et sur une trousse permettant la mise en oeuvre dudit procédé.

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