Method for recloning production cells

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 5/071 (2010.01) C12P 21/00 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2536239

The invention relates to a novel method for selecting clones and recloning mammal cells that are used in the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention also relates to methods for depositing and multiplying individual cell clones of the corresponding cells, to methods for producing proteins using cells that are obtained and multiplied by means of individual cell deposition, and to compositions that enable individual cells to be multiplied.

L'invention concerne un nouveau procédé de sélection clonale et de reclonage de cellules mammifères importantes dans l'élaboration de produits biopharmaceutiques, de préférence des cellules myélomateuses de souris ou de hamster, lequel procédé présente un degré d'automatisation et un rendement élevés. Cette invention concerne également des procédés de dépôt et de multiplication de clones de cellules isolées des cellules correspondantes. Ladite invention se rapporte en outre à des procédés de production de protéines au moyen de cellules, obtenues par le dépôt de cellules isolées puis multipliées, ainsi qu'à des compositions permettant une multiplication de cellules isolées.

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