C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 1/113 (2006.01) C07K 1/16 (2006.01) C07K 1/18 (2006.01) C07K 1/20 (2006.01) C07K 1/22 (2006.01) C07K 1/36 (2006.01)
Patent
CA 2462232
In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.
L'invention porte sur un procédé de reconstitution sous sa forme active d'une protéine de recombinaison dénaturée selon lequel: (a) une solution d'apport contenant la protéine de recombinaison sous sa forme intermédiaire dénaturée et/ou biologiquement inactive est soumise à un procédé chromatographique de séparation où la protéine est reconstituée dans des conditions provoquant un nouveau repliement, et (b) on sépare les formes intermédiaire des protéines re-repliées. Les formes intermédiaires dénaturées et/ou inactives séparés des protéines re-repliées, en continu ou quasiment en continu, sont facultativement recyclées vers la solution d'apport.
Jungbauer Alois
Machold Christine
Necina Roman
Schlegl Robert
Boehringer Ingelheim Austria Gmbh
Boehringer Ingelheim Rcv Gmbh & Co Kg
Fetherstonhaugh & Co.
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