Method for recovering a basic amino acid from a fermentation...

C - Chemistry – Metallurgy – 12 – P

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C12P 13/04 (2006.01) C07C 227/40 (2006.01) C07C 229/02 (2006.01)

Patent

CA 2604781

The invention relates to a method for recovering a basic amino acid from the fermentation liquor of a micro-organism strain that produces the basic amino acid. The method comprises the following steps: a) isolation of the micro- organisms from the fermentation liquor and b) separation of the basic amino acid from the aqueous liquor that has been obtained in step a) by the successive charging of a single or multiple stage arrangement of a strongly acidic cation exchanger in the form of a salt with the liquor obtained in step a) and the elution of the basic amino acid. According to the invention, prior to the charging process in step b), the aqueous liquor has a pH value ranging between 4 and 7.5 and at least the first stage of the cation exchanger arrangement is pre-treated with an aqueous acid in such a way that at the end of said pre-treatment, the pH value at the discharge of the pre-treated cation exchanger ranges between 4.5 and 7.

La présente invention concerne un procédé pour extraire un acide aminé basique du bouillon de fermentation d'une souche de micro-organisme produisant l'acide aminé basique. Ce procédé consiste a) à séparer les micro-organismes du bouillon de fermentation et b) à séparer l'acide aminé basique du bouillon aqueux obtenu à l'étape a), en chargeant successivement un ensemble mono- ou multiétagé d'un échangeur de cations fortement acide sous forme ionique en bouillon obtenu à l'étape a) puis en éluant l'acide aminé basique, le bouillon aqueux présentant, avant l'étape de charge b), un pH compris entre 4 et 7,5 et au moins le premier étage de l'ensemble échangeur de cations étant prétraité avec un acide aqueux, de sorte qu'à la fin du prétraitement, le pH de l'effluent de l'échangeur de cations prétraité est compris entre 4,5 et 7.

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