Method for recovery of .alpha.-l-aspartyl-l-phenylalanine...

C - Chemistry – Metallurgy – 07 – K

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C07K 5/075 (2006.01) C07C 227/40 (2006.01) C07C 227/42 (2006.01) C07C 229/24 (2006.01) C07C 229/36 (2006.01) C07K 1/14 (2006.01) C07K 5/072 (2006.01)

Patent

CA 2075307

The recovery of .alpha.-L-aspartyl-L-phenylalanine methyl ester, L-phenylalanine and L-aspartic acid from a mother liquor obtained by solid-liquid separation of a suspension of .alpha.-L-aspartyl-L-phenylalanine methyl ester crystals, is carried out by concentrating part or all of the mother liquor without desalting to provide a non-desalted concentrate; either adding a mineral acid and methanol to a part of the non-desalted concentrate or mixing a part of the non-desalted concentrate with a solvent comprising a mineral acid, methanol, water and, optionally, .alpha.-L-aspartyl-L-phenylalanine methyl ester, to precipitate an acid addition salt of .alpha.-L-aspartyl-L- phenylalanine methyl ester; recovering .alpha.-L-aspartyl-L- phenylalanine methyl ester as its acid salt; neutralizing the acid salt and recovering .alpha.-L-aspartyl-L-phenylalanine methyl ester; hydrolyzing the remaining concentrate with a mineral acid: and recovering L-phenylalanine or L-phenylalanine and L-aspartic acid.

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