Method for reducing expression variability of transgenes in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01)

Patent

CA 2262815

A method of making recombinant plant cells having reduced variability of expression of foreign genes therein is described herein. The method comprises (a) providing a plant cell capable of regeneration; and (b) transforming the plant cell with a DNA construct comprising an expression cassette, which construct comprises a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and an insulator (or "boundary element") positioned (i) 5' to the trancrisption initiation region, (ii) 3' to the structural gene, or (iii) both 5' to the transcription initiation region and 3' to the structural gene. DNA constructs useful for carrying out the method and plant cells and plants produced by the method are also disclosed.

L'invention se réfère à un procédé permettant de produire des cellules végétales recombinantes dotées d'une variabilité réduite de l'expression de gènes étrangers. Le procédé comprend (a) l'obtention d'une cellule végétale susceptible de régénération; et (b) la transformation de la cellule végétale au moyen d'une structure d'ADN comportant une cassette d'expression, ladite structure comportant une zone d'initiation de transcription, un gène de structure situé en aval de la zone d'initiation de transcription et qui est associé fonctionnellement à celle-ci, et un isolant (ou élément de limite) situé (i) en position 5' par rapport à la zone d'initiation de transcription, (ii) en position 3' par rapport au gène de structure, ou (iii) en position 5' par rapport à la zone de transcription et en position 3' par rapport au gène de structure. Ladite invention se réfère également à des structures d'ADN utiles pour réaliser le procédé, des cellules végétales et des plantes produites selon ledit procédé.

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