Method for reducing expression variability of transgenes in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2292842

A method of reducing gene silencing, increasing expression, and/or reducing expression variability of foreign DNA in plants or plant cells comprises providing a plant cell capable of regeneration; and then transforming said plant cell with a DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a first matrix attachment region, a transcription initiation region, a structural gene positioned downstream from said transcription initiation region and operatively associated therewith, and a second matrix attachment region, wherein said first and second matrix attachment regions are different.

L'invention concerne un procédé qui permet de réduire le silence génique, d'intensifier l'expression et/ou d'atténuer la variabilité d'expression d'un ADN étranger dans les végétaux ou les cellules végétales. Selon le procédé, on produit une cellule végétale capable de régénération; puis on la transforme en utilisant une construction d'ADN qui renferme une cassette d'expression et qui comprend, dans le sens 5' vers 3', une première région de fixation de matrice, une région de mise en route de transcription, un gène de structure positionné en aval de ladite région de mise en route de transcription et fonctionnellement associée avec elle, et une seconde région de fixation de matrice, lesdites première et seconde région de fixation de matrice étant différentes.

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