C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2220270
The present invention relates to a method for reducing the amount of substances inhibitory to nucleic acid hybridization in samples. The method is practiced prior to release of target nucleic acid from cells of interest and involves contacting the sample with an agent which solubilizes the inhibitory substances and does not effectuate release of nucleic acids from cells in the sample, and then the cells from the agent
Cette invention porte sur une méthode servant à réduire la quantité de substances inhibant l'hybridation de l'acide nucléique dans les échantillons. Cette méthode est pratiquée avant la libération de l'acide nucléique cible des cellules jugées intéressantes; selon cette méthode, on met d'abord l'échantillon en contact avec un agent qui solubilise d'abord les substances inhibitrices sans provoquer la libération des acides nucléiques des cellules de l'échantillon, puis les cellules de l'agent.
Collis Matthew P.
Little Michael C.
Llorin Oscar J.
Becton Dickinson And Company
Gowling Lafleur Henderson Llp
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