Method for reducing phytate in canola meal using genetic...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A23K 1/165 (2006.01) C12N 9/90 (2006.01) C12N 15/11 (2006.01) C12N 15/61 (2006.01)

Patent

CA 2375071

A plant, multicellular fragment of said plant or seed of said plant transformed with a nucleotide sequence of SEQ ID NO 1 or an allelic variant or a fragment thereof or a genetic equivalent thereof according to the degeneracy of the genetic code coding for a peptide having a <u>Brassica</u> myo-inositol 1-phosphate synthase activity, said plant, multicellular fragment or seed having reduced myo-inositol 1-phosphate synthase activity when compared with an equivalent untransformed plant, multicellular fragment or seed, such that there is reduced phytate present in the plant, multicellular fragment or seed. The invention also provides a method for reducing phytate in <u>Brassica</u>, which method comprises growing a <u>Brassica</u> plant comprising one of a myo- inositol 1-phosphate synthase antisense sequence and a myo-inositol 1- phosphate synthase cosuppression sequence thereby yielding a reduced amount of myo-inositol 1-phosphate synthase and consequently reduced phytate in said <u>Brassica</u>.

L'invention concerne une plante, un fragment multicellulaire de ladite plante ou la semence de ladite plante transformée par une séquence de nucléotide de SEQ ID NO 1 ou un allèle ou encore un fragment de celui-ci ou un équivalent génétique de celui-ci conformément à l'abâtardissement du code génétique codant pour un peptide comprenant une activité <u>Brassica</u> myo-inositol 1-phosphate synthase, ladite plante, le fragment multicellulaire ou la semence ayant réduit l'activité myo-inositol 1-phosphate synthase lorsqu'elle est comparée à une plante non-transformée équivalente, un fragment multicellulaire ou une semence ce cette dernière, telle qu'il existe des phytates réduits dans cette plante, ce fragment multicellulaire ou cette semence. L'invention concerne également un procédé pour réduire les phytates dans <u>Brassica</u>. Ce procédé comprend la croissance d'une plante <u>Brassica</u> comprenant une séquence anti-sens myo-inositol 1-phosphate synthase et/ou une séquence de cosuppression myo-inositol 1-phosphate synthase qui permet de produire une quantité réduite de myo-inositol 1-phosphate synthase et en conséquence des phytates réduits dans ladite <u>Brassica</u>.

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