Method for reduction of transgene copy number

Details Method for reduction of transgene copy number Method for reduction of transgene copy number

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2315166

The present invention provides methods for the efficient production of transformants with low transgene copy numbers. In the method, the average transgene copy number of transformants is decreased through methods which are believed to limit the interaction between segments of transforming DNA prior to transformation. The methods comprise means for end-modification of transforming DNA and use of limited quantities of DNA for transformation. Production of single or low copy transformation events is desirable in that it avoids many of the problems associated with high transgene copy number including co-suppression, unpredictable gene expression and non-Mendelian inheritance.

L'invention porte sur des procédés efficaces de production de transformants à l'aide d'un nombre peu élevé de copies de transgènes, ce nombre peu élevé étant obtenus par des techniques censées limiter les interactions entre segments d'ADN transformateur avant la transformation. Ces techniques incluent des moyens de modification finale de l'ADN transformateur et l'utilisation de quantités limitées d'ADN pour les transformations. La production d'une seule copie ou de peu de copies d'événements de transformation est préférable en ce qu'elle évite nombre de problèmes associés au nombre élevé de copies de transgènes, dont la co-suppression, l'expression de gènes imprévisibles ou les héritages non Mendéliens.

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