Method for refolding igf-1 to active conformation

Details Method for refolding igf-1 to active conformation Method for refolding igf-1 to active conformation

C - Chemistry, Metallurgy

12

N

C12N 15/16 (2006.01) C07K 1/107 (2006.01) C07K 1/113 (2006.01) C07K 14/65 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2122837

A method for refolding insoluble, improperly folded IGF-I is provided, wherein the IGF-I, precipitated from prokaryotic host cells, is concurrently solubilized, unfolded, and refolded into a biologically active conformation in a single buffer. The buffer contains reducing agent and chaotropic agent to solubilize the IGF-1 at concentrations sufficiently low to allow solubilization and refolding to occur. Also provided is a triple-protease deficient E. coli host suitable for use in the process.

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