Method for relative quantification of attached nucleic acids

Details Method for relative quantification of attached nucleic acids Method for relative quantification of attached nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2433674

A method and associated compositions for the relative quantification of nucleic acid on an address-defined surface, involving fitting the nucleic acid with a generic oligonucleotide, and hybridizing the generic oligonucleotide with a directly or indirectly labeled complementary oligonucleotide. The method is applicable, for example, to SNP genotyping and gene expression analysis.

La présente invention concerne une méthode et des compositions associées qui permettent d'effectuer la quantification relative d'acide nucléique sur une surface définie par une adresse. Dans cette méthode, on dote l'acide nucléique d'un oligonucléotide générique, puis on hybride l'oligonucléotide générique avec un oligonucléotide complémentaire marqué de manière directe ou indirecte. Cette méthode peut être appliquée, par exemple, au génotypage SNP (polymorphisme nucléotidique simple) et à l'analyse de l'expression génique.

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