Method for removing a universal linker from an oligonucleotide

Details Method for removing a universal linker from an oligonucleotide Method for removing a universal linker from an oligonucleotide

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2428155

The invention relates to a method for cleavage of a linker from an oligonucleotide comprising contacting an oligonucleotide-linker conjugate with a gaseous nucleophilic cleavage reagent under conditions that result in the cleavage of the linker from the oligonucleotide.

L'invention a trait à un procédé servant à séparer par clivage un lieur d'un oligonucléotide, et consistant à cet effet à mettre un conjugué lieur-oligonucléotide en contact avec un réactif de clivage nucléophile gazeux dans des conditions permettant le clivage du lieur de l'oligonucléotide.

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