Method for removing prion protein

G - Physics – 01 – N

Patent

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Details

G01N 33/68 (2006.01) A61L 2/00 (2006.01)

Patent

CA 2675039

The present invention relates to a method for removing prion PrPSc proteins from biological material by contacting a biological material comprising prion PrPSc proteins with sepharose under conditions that allow for the specific and high affinity binding of the sepharose to the prion PrPSc proteins and removing the biological material from the sepharose wherein the biological material is selected from mammalian urine or a fraction thereof or from cell culture-derived materials. Another aspect of the present invention concerns the use of specific and high affinity sepharose for removing prion PrPSc proteins from biological material.

L'invention porte sur un procédé qui permet d'enlever les protéines prions PrPSc d'une matière biologique en mettant en contact une matière biologique contenant des protéines prions PrPSc avec de la Sepharose dans des conditions qui permettent à la Sepharose de se lier de manière spécifique et avec une haute affinité aux protéines prions PrPSc, et en retirant la matière biologique de la Sepharose, la matière biologique étant choisie entre de l'urine mammalienne ou une fraction de cette dernière et des matières dérivées de cultures cellulaires. Un autre aspect de l'invention concerne l'utilisation de Sepharose à affinité spécifique et élevée afin d'enlever les protéines prions PrPSc de la matière biologique.

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