Method for removing tumor cells from tumor cell-contaminated...

A - Human Necessities – 61 – K

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A61K 35/14 (2006.01) A61K 35/28 (2006.01) A61M 1/34 (2006.01) A61M 1/36 (2006.01) B01D 39/16 (2006.01) B01D 39/18 (2006.01) C12M 3/06 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2261612

A method for removing tumor cells from tumor cell-contaminated stem cell products is disclosed. One embodiment of the method relies on an in-line filtration device that includes a tumor cell reduction filter means, preferably comprised of one or more tumor cell reduction filter pads. The tumor cell reduction filter means provides at least a ten-fold reduction in tumor cells while allowing for at least a 30 % recovery of stem cells in a filtered stem cell product.

On décrit un procédé d'enlèvement de cellules tumorales à partir de produits contenant des cellules souches et contaminés par des cellules tumorales. Dans un mode de réalisation du procédé, on utilise un dispositif de filtration direct comprenant un filtre de réduction de cellules tumorales, lequel présente, de préférence, un ou plusieurs tampons filtres de réduction des cellules tumorales. Ce filtre permet à la fois d'obtenir une réduction au moins multipliée par dix des cellules tumorales, et de récupérer au moins 30 % des cellules souches, dans un produit contenant ces cellules souches et filtré.

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