Method for rendering the membranes of biological cells...

A - Human Necessities – 23 – L

Patent

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Details

A23L 2/48 (2006.01) A23L 2/50 (2006.01) A23L 3/32 (2006.01) C02F 1/48 (2006.01) C12N 13/00 (2006.01)

Patent

CA 2755858

The invention relates to a method for rendering the membranes of biological cells in a product pervious, said method being implemented in a treatment device including at least one treatment chamber emitting a pulsed electric field, said method including the following steps: a step of supplying the treatment device with a product including said biological cells at a predetermined supply rate; a step of inserting the product including said biological cells into said treatment chamber at an insertion rate matching the aforementioned supply rate to which the withdrawal rate mentioned in the withdrawing step below is added; a step of treating said product inserted into said chamber by a pulsed electric field; a step of withdrawing, at a predetermined withdrawal rate, the product at the outlet of said chamber so as to carry said product back upstream from said chamber.

L' invention a trait à un procédé de perméabilisation membranaire de cellules biologiques contenues dans un produit, ledit procédé étant mis en oeuvre dans un dispositif de traitement comprenant au moins une chambre de traitement émettant un champ électrique puisé, ledit procédé comprenant les étapes suivantes : - une étape d'alimentation du dispositif de traitement en produit comprenant lesdites cellules biologiques à un débit d'alimentation prédéterminé; - une étape d' introduction du produit comprenant lesdites cellules biologiques dans ladite chambre de traitement à un débit d' introduction correspondant au débit d'alimentation susmentionné auquel s'ajoute le débit de soutirage mentionné à l'étape de soutirage ci-dessous; - une étape de traitement dudit produit introduit dans ladite chambre par un champ électrique puisé; - une étape de soutirage, à un débit de soutirage prédéterminé, du produit en sortie de ladite chambre de sorte à le réacheminer en amont de ladite chambre.

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