Method for replicating nucleic acids and novel unnatural...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C07H 19/044 (2006.01) C07H 19/23 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2642657

Disclosed are: a method for replication of a nucleic acid; and a novel artificial base pair. The method is characterized by using a deoxyribonucleoside 5'-phosphate having a hydroxyl group at .gamma.-position in the phosphate substituted by a group selected from the group consisting of an amino group, a methylamino group, a diemthylamino group, a mercapto group and a fluoro group as a substrate in a replication reaction. The novel artificial base pair is characterized in that a base pair is formed between 7-(2-thienyl)-imidazo[4,5-b]pyridine (Ds) or an analogue thereof and pyrrole-2-carbaldehyde (Pa) or an analogue thereof.

L'invention concerne : un procédé pour la réplication d'un acide nucléique ; et une nouvelle paire de bases artificielle. Le procédé est caractérisé en ce qu'il utilise en tant que substrat dans une réaction de réplication un désoxyribonucléoside 5'-phosphate ayant un groupe hydroxyle en position .gamma. dans le phosphate substitué par un groupe sélectionné dans le groupe constitué d'un groupe amino, d'un groupe méthylamino, d'un groupe diméthylamino, d'un groupe mercapto et d'un groupe fluoro. La nouvelle paire de bases artificielle est caractérisée en ce qu'une paire de bases est formée entre la 7-(2-thiényl)imidazo[4,5-b]pyridine (Ds) ou un analogue de celle-ci et le pyrrole-2-carbaldéhyde (Pa) ou un analogue de celui-ci.

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