A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
47/4, 195/47
A01H 4/00 (2006.01)
Patent
CA 2028855
The present invention is a method for reproducing coniferous trees by somatic embryogenesis using plant tissue culture techniques. The method comprises a multistage culturing process. A suitable explant, typically the fertilized embryo excised from a mature or imma- ture seed, is first cultured on a medium that induces multiple early stage proembryos. Preferably the proembryos from the induction stage are further multiplied in a second culture having reduced growth hor- mones. The early stage proembryos are then placed in or on a late stage proembryo development culture having a significantly higher osmotic potential than the previous stage or stages. This increased osmotic potential medium is a critical key to the development of very robust late stage proembryos having at least about 100 cells and multiple suspensor cells. Culturing from this point continues in an embryo devel- opment medium very low in or lacking growth hormones but containing abscisic acid. After a period of several weeks cotyledonary embryos will have formed. These have a well defined bipolar structure with cotyledonary primordia at one end and a latent radicle at the other. Culturing to this point is carried out in darkness or greatly subdued light. The cotyledonary embryos are then transferred to a growth medium with a light/dark photoperiod for development of plantlets. The plant- lets may then be transplanted to soil for further growth. The method has been successful with a broad range of species and with numerous genotypes that could not previously be propagated by embryogenesis.
Gupta Pramod Kumar
Pullman Gerald Steven
Smart & Biggar
Weyerhaeuser Nr Company
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