Method for screening agent acting on cell wall

Details Method for screening agent acting on cell wall Method for screening agent acting on cell wall

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 1/19 (2006.01) C12Q 1/02 (2006.01) C12Q 1/18 (2006.01)

Patent

CA 2407906

A method of screening a drug acting on cell wall which involves: (1) the step of incubating microorganisms, in which a reporter protein is fixed as GPI anchor protein respectively to the cell wall and the cell membrane, in the presence of a test substance; (2) the step of assaying the reporter protein released in the cultures of the above microorganisms; and (3) the step of judging the test substance as a drug having a selective inhibitory action on the cell wall, in case where the reporter protein is released into the culture of the microorganism having the reporter protein fixed to the cell wall but not substantially released into the culture of the other microorganism having the reporter protein fixed to the cell membrane.

La présente invention concerne un procédé de criblage d'un médicament qui agit sur la paroi cellulaire, consistant à: (1) incuber des micro-organismes dans lesquels une protéine rapporteur est fixée comme une protéine d'ancrage GPI et ce, respectivement au niveau de la paroi cellulaire et de la membrane cellulaire, en présence d'une substance d'essai; (2) doser la protéine rapporteur libérée dans les cultures des micro-organismes précités; et (3) évaluer la substance d'essai comme un médicament présentant une action inhibitrice sélective sur la paroi cellulaire, dans le cas où la protéine rapporteur est libérée dans la culture du micro-organisme dans lequel la protéine rapporteur est fixée sur la paroi cellulaire, mais pratiquement pas libérée dans la culture de l'autre micro-organisme dans lequel la protéine rapporteur est fixée sur la membrane cellulaire.

Affiliated with

Also associated with

Rating

Method for screening agent acting on cell wall does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for screening agent acting on cell wall, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for screening agent acting on cell wall will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1863495