Method for screening crystallization conditions in solution...

G - Physics – 01 – N

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G01N 31/00 (2006.01) G01N 25/14 (2006.01) G01N 33/00 (2006.01)

Patent

CA 2369935

A method of screening protein crystal growth conditions with picogram to microgram amounts of protein in picoliter or nanoliter volumes is provided. A preferred method comprises a microarray with a plurality of micro-chambers in the microarray. A protein solution is placed into the micro-chambers by an automated dispensing mechanism. The protein crystal growth conditions of each of the micro-chambers is adjusted so that the protein crystal growth conditions in at least two of the micro-chambers differs. Crystallization of the protein solution in the micro-chambers is effected. Protein crystal growth in the micro-chambers is then observed.

La présente invention concerne un procédé de criblage des conditions de tirage de cristaux de protéine en des quantités de picogrammes à microgrammes de protéine en des volumes de picolitres ou nanolitres. Dans un mode de réalisation préféré, le procédé comprend un jeu ordonné de microéchantillons avec une pluralité de micro-cavités dans le jeu ordonné de microéchantillons. Une solution de protéine est placée dans les micro-cavités au moyen d'un mécanisme de distribution automatisé. Les conditions de tirage de cristaux de protéine dans chaque micro-cavité sont réglées de sorte que les conditions de tirage de cristaux de protéine sont différentes dans au moins deux micro-cavités. La cristallisation de la solution de protéine est effectuée dans les micro-cavités. On observe ensuite le tirage de cristaux de protéine.

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