Method for screening for receptor agonists

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) A61K 31/445 (2006.01) C07K 14/72 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2160135

Method for assay for a receptor agonist. The method includes the steps of: providing nucleic acid encoding a receptor having a first TAF region able to activate transcription from a promotor, and a second TAF region mutated to have the functional context of the second TAF region, but not able on its own to activate transcription of the promoter. The nucleic acid is provided within a cell unable to exhibit transcription from the promoter in the presence of the second TAF region alone, but able to exhibit transcription from the promoter in the presence of the first TAF region. The cell further includes a reporter construct containing the promoter. The reporter construct is transcribed when the promoter is activated in the presence of the first TAF region. The method further includes contacting the cell with a potential agonist, under conditions in which contact of the cell with a known agonist of the receptor causes transcription from the promoter, and increases the level of the product of the reporter construct. Finally, the method involves measuring the level of increase of the product of the reporter construct as an indication of the agonist activity of the potential agonist.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Method for screening for receptor agonists does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for screening for receptor agonists, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for screening for receptor agonists will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-2080272

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.